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21/09/2019 7:06 am
Effect of caffeine on metabolism of L-arginine in the brain
Authors: Nikolic J.1; Bjelakovic G.2; Stojanovic I.2
Source: Molecular and Cellular Biochemistry, Volume 244, Number 1, February 2003 , pp. 125-128(4)
Publisher: Springer
Methylxanthines are widely consumed because of their stimulating effect primarily on the central nervous system. Their diuretic and respiratory stimulant action is used in clinical medicine. L-Arginine metabolism in the brain is very important for normal brain function. In addition to brain protein synthesis, arginine is a substrate for the production of urea, creatine, nitric oxide, agmatine, glutamic acid, ornithine, proline and polyamines. As known, many of these compounds are very important in brain function. There is no information relating to effects of caffeine on arginine metabolism in the brain, however, there is a lot of new information about arginine metabolism and caffeine action on the central nervous system. So, we have hypothesized the existence of a relationship that may be of interest in understanding mechanisms of caffeine effects on the central nervous system that may have utility in the clinical applications.
In our experiment protocol we used male Wistar rats weighing about 200 g. Caffeine was added to the drinking water in gradually increasing amounts, from 2 g/l over the first 3 days, to 4 g/l over the last 7 days. A control group was given drinking water without caffeine. The level of lipid peroxidation, arginase and diamine oxidase (DAO) activity in the brain was measured. The results of our study show that arginase and diamine oxidase were decreased in animals treated with caffeine. The level of lipid peroxidation (MDA) was decreased also.
The inhibitory effect of caffeine on arginase activity indicates that caffeine provides more arginine for consumption in other metabolic pathways. Considering the central stimulant effects of caffeine and the decreased lipid peroxidation level, it can be assumed that moderate short-term consumption of caffeine may be beneficial for brain function.
21/09/2019 7:33 am
we all knew that!
jb
Topic starter
21/09/2019 7:56 am
It's the arginine involvement that I found of interest. It's a bit of a topic for me at the moment.
21/09/2019 8:21 am
so you must be thinking about l-citrulline?
jb
Topic starter
21/09/2019 8:44 am
citrulline malate and di-arginine malate
21/09/2019 9:13 am
i use both l-citrulline and l-citrulline malate daily.
jb
Topic starter
23/09/2019 8:32 am
I'm "running" beta-alanine, L-citrulline malate, di-arginine malate, CEE, GEE, ALCAR, r-ALA, glucorolactone, taken first thing a.m. and pre-workout, as well as nootropics piracetam, ginkgo, hydergine, selegiline as a post PCT sup-stack-bridge.
Aiming for blood flow "pump" for nutritional metabolic optimisation within the muscle.
Also taking whey, MPC, milk thistle conc ex, green tea conc ex, Grape seed conc ex and vits.
23/09/2019 8:59 am
add nac. I always left out piracetam while taking hydergine. Also why not add vinpocetine since it is available domestically in bulk cheap.
jb
Topic starter
23/09/2019 9:21 am
i use both l-citrulline and l-citrulline malate daily.jb
Why both?
Topic starter
23/09/2019 9:51 am
add nac. I always left out piracetam while taking hydergine. Also why not add vinpocetine since it is available domestically in bulk cheap.jb
NAC just for liver? What other benefits do you find it gives?
I like the hydergine ginkgo combo as the ginkgo adds a certain focus which I find lacking in hydergine and hydergine with piracetam I find rather scatty ie too much thought to directionless, so the combination of all 3 seems to work quite well, for me anyways. I have yet to try sulbutiamine as an alternative to hydergine.
I do like vincamine, never tried vinpocetine. How do you find they compare? but/and I'm not US domestic (I'm Europe based, mainly the UK) so I often incur an importation tax, unless it's sent as a gift which some companies won't do.
23/09/2019 10:15 am
ic, i find the vinpocetine seems to make things clearer. I know that is pretty vague. NAC is a valuable supp not only for liver protection but for protection across a broad spectrum of issues. some are:
N-Acetyl Cysteine increases Glutathione Levels
N-Acetyl Cysteine Chelates Heavy Metals
N-Acetyl Cysteine boosts the Immune System
N-Acetyl Cysteine Breaks up Mucus
there are over 7k references to it with ~500 title refs in medline.
jb
Topic starter
23/09/2019 10:40 am
Thanks for that jb. Could you tell me what is your experience of NAC please?
23/09/2019 11:08 am
I just use NAC at 400-600 mg/day!
"The medals don't mean anything and the glory doesn't last. It's all about your happiness. The rewards are going to come, but my happiness is just loving the sport and having fun performing" ~ Jackie Joyner Kersee.
23/09/2019 11:29 am
i just add it as a general body system protection agent. it is cheap and very effective in helping/preventing a wide variety of problems.
400-600 is a good place to start.
jb
Topic starter
23/09/2019 11:57 am
Thanks for the chime quijr.
On order jb. Thanks for the info and direction, much appreciated.