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Topic starter
02/04/2020 8:02 pm
As AICAR is so expensive that even Bill Gates would think twice about using it, AMPK activation could be done via a much cheaper CLA/metformin stack instead:
Conjugated Linoleic Acid Activates AMP-Activated Protein Kinase and Reduces Adiposity More Effectively When Used with Metformin in Mice.
J Nutr. 2009 Oct 14. [Epub ahead of print]
Jiang S, Wang Z, Riethoven JJ, Xia Y, Miner J, Fromm M.
Department of Animal Science, University of Nebraska, Lincoln, NE 68502.
Trans-10, cis-12 (t10c12) conjugated linoleic acid (CLA) reduces lipid levels in adipocytes, but the mechanisms involved are still emerging. The hypotheses of this study were that t10c12 CLA treatment activated AMP-activated protein kinase (AMPK) and that the effectiveness of a low dose of t10c12 CLA would be increased when combined with an AMPK activator. We demonstrated t10c12 CLA, directly or indirectly, activated AMPK and increased the amount of phosphorylated acetyl-CoA carboxylase (ACC) in 3t3-L1 adipocytes. Compound C, a potent inhibitor of AMPK, attenuated the phosphorylation of ACC, integrated stress response (ISR), inflammatory response, reduction in key lipogenic transcription factors, and triglyceride (TG) reduction that otherwise occurred in t10c12 CLA-treated adipocytes. Treatment of adipocytes or mice with a low dose of t10c12 CLA in conjunction with the AMPK activator metformin resulted in more TG loss than treatment with the individual chemicals. Additionally, although an inflammatory response was required for robust TG reduction, the combination of t10c12 CLA with AMPK activators had a similar TG loss with a reduced inflammatory response. A microarray analysis of the transcriptional response to either t10c12 CLA, metformin, or the combination, indicated the responses were very similar, with a correlation coefficient of 0.91 or better for genes in the ISR or lipid-related pathways. Altogether, these results support our hypotheses that t10c12 CLA activates AMPK, directly or indirectly, and that metformin increases the effectiveness of t10c12 CLA in reducing TG amounts in adipocytes.
02/04/2020 8:19 pm
I did a search on metformin on the forum and found that RealGains and JBoldman both said:
"Don't touch the shit bro!"
So...I would tend to follow the advise of both those Bros.
On the subject of CLA > I take CLA and Calcium+Vit D before every meal.
What does of CLA is best for fat loss when trying to lean out?
Cheers,
G/L
To most the marathon is the ultimate test of human endurance. To us it's just the cool down!
Pain is temporary - Quitting lasts forever
Regardless of how hard you train - The time is going to go by anyway so if you are going to throw a leg over your bike you may aswell bust you ass, make as many watts / do as many intervals as you can.
Topic starter
02/04/2020 8:38 pm
there is a risk of developing lactic acidosis with metformin, though that is in fat type 2 diabetic patients, don't know what the risk is if you have normal sugars & a physiological tolerance to lactate from threshold training! but I've read other stuff saying metformin negatively impacts endurance, so I guess its just the CLA & quercitin for now to boost AMPK!